Review



stimulation with atp 2mm  (InvivoGen)


Bioz Verified Symbol InvivoGen is a verified supplier
Bioz Manufacturer Symbol InvivoGen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    InvivoGen stimulation with atp 2mm
    A) Wild type (WT) and PIM triple knockout (TKO) BMDMs were stimulated with IFNγ (500 ng/ml) and LPS) (20ng/ml) for 24 hours or B) with IL-4 (20 ng/ml) for 24 hours, and lysates were collected for immunoblotting C) WT BMDMs were treated with PIM447 (3μM) and stimulated with IFNγ/LPS (M1) or IL-4 (M2). Lysates were analyzed by western blotting. D) WT BMDMs were pre-treated with PIM447 for 16 hours, followed by <t>stimulation</t> with LPS for 4 hours and <t>ATP</t> for 30 min to induce inflammasome activation. Lysates were analyzed by western blotting. E) Supernatant was collected from each cell condition and secreted IL-1β was quantified by ELISA. F) WT BMDM were pre-treated with PIM447, followed by LPS/ATP and immunoblotting was used to assess NF-κB proteins. G) mRNA analysis of TNFα.*p<0.05 Error bars = SEM.
    Stimulation With Atp 2mm, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 319 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stimulation with atp 2mm/product/InvivoGen
    Average 96 stars, based on 319 article reviews
    stimulation with atp 2mm - by Bioz Stars, 2026-02
    96/100 stars

    Images

    1) Product Images from "Inhibition of PIM kinase in tumor associated macrophages suppresses inflammasome activation and sensitizes prostate cancer to immunotherapy"

    Article Title: Inhibition of PIM kinase in tumor associated macrophages suppresses inflammasome activation and sensitizes prostate cancer to immunotherapy

    Journal: bioRxiv

    doi: 10.1101/2024.10.21.618756

    A) Wild type (WT) and PIM triple knockout (TKO) BMDMs were stimulated with IFNγ (500 ng/ml) and LPS) (20ng/ml) for 24 hours or B) with IL-4 (20 ng/ml) for 24 hours, and lysates were collected for immunoblotting C) WT BMDMs were treated with PIM447 (3μM) and stimulated with IFNγ/LPS (M1) or IL-4 (M2). Lysates were analyzed by western blotting. D) WT BMDMs were pre-treated with PIM447 for 16 hours, followed by stimulation with LPS for 4 hours and ATP for 30 min to induce inflammasome activation. Lysates were analyzed by western blotting. E) Supernatant was collected from each cell condition and secreted IL-1β was quantified by ELISA. F) WT BMDM were pre-treated with PIM447, followed by LPS/ATP and immunoblotting was used to assess NF-κB proteins. G) mRNA analysis of TNFα.*p<0.05 Error bars = SEM.
    Figure Legend Snippet: A) Wild type (WT) and PIM triple knockout (TKO) BMDMs were stimulated with IFNγ (500 ng/ml) and LPS) (20ng/ml) for 24 hours or B) with IL-4 (20 ng/ml) for 24 hours, and lysates were collected for immunoblotting C) WT BMDMs were treated with PIM447 (3μM) and stimulated with IFNγ/LPS (M1) or IL-4 (M2). Lysates were analyzed by western blotting. D) WT BMDMs were pre-treated with PIM447 for 16 hours, followed by stimulation with LPS for 4 hours and ATP for 30 min to induce inflammasome activation. Lysates were analyzed by western blotting. E) Supernatant was collected from each cell condition and secreted IL-1β was quantified by ELISA. F) WT BMDM were pre-treated with PIM447, followed by LPS/ATP and immunoblotting was used to assess NF-κB proteins. G) mRNA analysis of TNFα.*p<0.05 Error bars = SEM.

    Techniques Used: Triple Knockout, Western Blot, Activation Assay, Enzyme-linked Immunosorbent Assay



    Similar Products

    stimulation with atp 2mm  (InvivoGen)
    96
    InvivoGen stimulation with atp 2mm
    A) Wild type (WT) and PIM triple knockout (TKO) BMDMs were stimulated with IFNγ (500 ng/ml) and LPS) (20ng/ml) for 24 hours or B) with IL-4 (20 ng/ml) for 24 hours, and lysates were collected for immunoblotting C) WT BMDMs were treated with PIM447 (3μM) and stimulated with IFNγ/LPS (M1) or IL-4 (M2). Lysates were analyzed by western blotting. D) WT BMDMs were pre-treated with PIM447 for 16 hours, followed by <t>stimulation</t> with LPS for 4 hours and <t>ATP</t> for 30 min to induce inflammasome activation. Lysates were analyzed by western blotting. E) Supernatant was collected from each cell condition and secreted IL-1β was quantified by ELISA. F) WT BMDM were pre-treated with PIM447, followed by LPS/ATP and immunoblotting was used to assess NF-κB proteins. G) mRNA analysis of TNFα.*p<0.05 Error bars = SEM.
    Stimulation With Atp 2mm, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stimulation with atp 2mm/product/InvivoGen
    Average 96 stars, based on 1 article reviews
    stimulation with atp 2mm - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    atp  (InvivoGen)
    99
    InvivoGen atp
    A) Wild type (WT) and PIM triple knockout (TKO) BMDMs were stimulated with IFNγ (500 ng/ml) and LPS) (20ng/ml) for 24 hours or B) with IL-4 (20 ng/ml) for 24 hours, and lysates were collected for immunoblotting C) WT BMDMs were treated with PIM447 (3μM) and stimulated with IFNγ/LPS (M1) or IL-4 (M2). Lysates were analyzed by western blotting. D) WT BMDMs were pre-treated with PIM447 for 16 hours, followed by <t>stimulation</t> with LPS for 4 hours and <t>ATP</t> for 30 min to induce inflammasome activation. Lysates were analyzed by western blotting. E) Supernatant was collected from each cell condition and secreted IL-1β was quantified by ELISA. F) WT BMDM were pre-treated with PIM447, followed by LPS/ATP and immunoblotting was used to assess NF-κB proteins. G) mRNA analysis of TNFα.*p<0.05 Error bars = SEM.
    Atp, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atp/product/InvivoGen
    Average 99 stars, based on 1 article reviews
    atp - by Bioz Stars, 2026-02
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    A) Wild type (WT) and PIM triple knockout (TKO) BMDMs were stimulated with IFNγ (500 ng/ml) and LPS) (20ng/ml) for 24 hours or B) with IL-4 (20 ng/ml) for 24 hours, and lysates were collected for immunoblotting C) WT BMDMs were treated with PIM447 (3μM) and stimulated with IFNγ/LPS (M1) or IL-4 (M2). Lysates were analyzed by western blotting. D) WT BMDMs were pre-treated with PIM447 for 16 hours, followed by stimulation with LPS for 4 hours and ATP for 30 min to induce inflammasome activation. Lysates were analyzed by western blotting. E) Supernatant was collected from each cell condition and secreted IL-1β was quantified by ELISA. F) WT BMDM were pre-treated with PIM447, followed by LPS/ATP and immunoblotting was used to assess NF-κB proteins. G) mRNA analysis of TNFα.*p<0.05 Error bars = SEM.

    Journal: bioRxiv

    Article Title: Inhibition of PIM kinase in tumor associated macrophages suppresses inflammasome activation and sensitizes prostate cancer to immunotherapy

    doi: 10.1101/2024.10.21.618756

    Figure Lengend Snippet: A) Wild type (WT) and PIM triple knockout (TKO) BMDMs were stimulated with IFNγ (500 ng/ml) and LPS) (20ng/ml) for 24 hours or B) with IL-4 (20 ng/ml) for 24 hours, and lysates were collected for immunoblotting C) WT BMDMs were treated with PIM447 (3μM) and stimulated with IFNγ/LPS (M1) or IL-4 (M2). Lysates were analyzed by western blotting. D) WT BMDMs were pre-treated with PIM447 for 16 hours, followed by stimulation with LPS for 4 hours and ATP for 30 min to induce inflammasome activation. Lysates were analyzed by western blotting. E) Supernatant was collected from each cell condition and secreted IL-1β was quantified by ELISA. F) WT BMDM were pre-treated with PIM447, followed by LPS/ATP and immunoblotting was used to assess NF-κB proteins. G) mRNA analysis of TNFα.*p<0.05 Error bars = SEM.

    Article Snippet: For inflammasome activation, BMDMs were treated with LPS (500ng/ml) (Invivogen) for 4 hours, followed by stimulation with ATP (2mM) (Invivogen) for 30 minutes.

    Techniques: Triple Knockout, Western Blot, Activation Assay, Enzyme-linked Immunosorbent Assay